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Individual haplotyping of whale sharks from seawater environmental DNA

Created 23/06/2025

Updated 23/06/2025

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Water samples were collected over two weeks directly behind individual whale sharks at Ningaloo Reef, Australia in May, 2019. A total of 56 seawater samples were collected, filtered immediately through sterile 0.22 μm filters, and stored at -80°C. Tissue biopsy samples were then taken from the same animal using a hand spear with a dart head, totalling 77 individual samples. Individual whale sharks were identified by their unique spot and stipe patters through photographs analysed by I³S Classic Software. Re-sightings of the same individual were removed from the dataset. Following photo-identification, a total of 48 unique individuals were confirmed, of which 42 consisted of both tissue and seawater samples. Additional samples were later discarded from the study, resulting in libraries for 28 seawater samples, and whale shark d-loop haplotypes. DNA extractions of the tissue samples were carried out and PCR amplification of the mitochondrial control region and reaction protocol verified. Seawater samples were processed and environmental DNA was extracted. Statistical analysis was performed with R Studio.

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Additional Info

Field Value
Title Individual haplotyping of whale sharks from seawater environmental DNA
Language eng
Licence Not Specified
Landing Page https://data.gov.au/data/en/dataset/95737b33-05f4-49c0-9693-f2bb83a4765b
Contact Point
Australian Ocean Data Network
reception@aims.gov.au
Reference Period 23/08/2021
Geospatial Coverage
Map data © OpenStreetMap contributors
{
  "coordinates": [
    113.6138,
    -22.643081
  ],
  "type": "Point"
}
Data Portal data.gov.au

Data Source

This dataset was originally found on data.gov.au "Individual haplotyping of whale sharks from seawater environmental DNA". Please visit the source to access the original metadata of the dataset:
https://data.gov.au/data/dataset/individual-haplotyping-of-whale-sharks-from-seawater-environmental-dna