UV-absorbing pigments in eye tissues of fish from the Great Barrier Reef

Created 24/06/2025

Updated 24/06/2025

Ocular tissues from 52 species (19 families) of tropical marine fish were examined for levels of UV-absorbing pigments in the spectral region 300-380 nm.Specimens of mackerels, tuna and marlin were caught by surface trolling in Bowling Green and Upstart Bays; all other fish were caught by hand-line or spearfishing at Grub, Hunt and Marion Reefs. The eyes were removed and frozen immediately at -20°C. Lens ocular tissues were excised from defrosted samples, crushed and extracts formed by steeping in 5ml methanol. Visible-u.v. absorption spectra from these extracts were recorded. Solid protein biomass was determined from the solid tissue residues. Mycosporine-like amino acids (Asterina-330, palythene, palythine and palythinol) were separated and quantified (nmol/mg protein) using reverse-phase isocratic high-performance liquid chromatography (HPLC).The efficiency of methanol extraction This research was undertaken to:1. identify and describe the distribution of mycosporine-like amino acid compounds in the lens tissues of marine fish2. develop techniques for quantitative studies of UV absorbing compounds in fish eyes using high performance liquid chromotography3. evaluate the efficiency with which these compounds can be extracted from lens tissues

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Additional Info

Field Value
Title UV-absorbing pigments in eye tissues of fish from the Great Barrier Reef
Language eng
Licence Not Specified
Landing Page https://data.gov.au/data/dataset/63d3d3f2-6316-4992-9b6c-ae3366b5170a
Contact Point
Australian Ocean Data Network
reception@aims.gov.au
Reference Period 20/11/2017
Geospatial Coverage
Map data © OpenStreetMap contributors
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Data Portal Australian Oceans Data Network

Data Source

This dataset was originally found on Australian Oceans Data Network "UV-absorbing pigments in eye tissues of fish from the Great Barrier Reef". Please visit the source to access the original metadata of the dataset:
https://catalogue.aodn.org.au/geonetwork/srv/eng/csw/dataset/uv-absorbing-pigments-in-eye-tissues-of-fish-from-the-great-barrier-reef1