From Australian Oceans Data Network
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Population genetics of the fissiparous Holothurian, Stichopus chloronotus, on the Great Barrier Reef

Created 23/06/2025

Updated 23/06/2025

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Population genetic structure was studied in one nearshore (Great Palm Island) and two offshore (Rib Reef and Reef 18-026) populations of Stichopus chloronotus on the Great Barrier Reef. Two stations approximately 500m were sampled on each reef at depths between 1 and 2 m. At each station, 30-40 individuals were sampled within a radius of 30m, in early November 1996. The sampling time in November was chosen to increase the likelihood of finding mature gonads in the sampled animals.Genetic variation at 5 polymorphic loci was examined using allozyme electrophoresis. The polymorphic enzymes examined were: hexokinase (E.C. 2.7.1.1; HK), mannose- 6-phosphate isomerase (E.C. 5.3.1.8; MPI), phosphoglucomutase (E.C. 5.4.2.2; PGM), triose-phosphate isomerase (E.C. 5.3.1.1; ¹PI) and peptidase using valylleucine as substrate (E.C. 3.4.11/13; Estimates of the level of asexual reproduction were made using the ratios of the number of sexually produced individuals to sample size, observed genotypic diversity (Go) to expected genotypic diversity (Ge), and number of genotypes (Ngo) to sample size. Values of Go/Ge much smaller than one indicate the occurrence of clonal reproduction.F-statistics were used to partition genetic variation into that occurring within populations (FIS) and that occurring between populations (FST). To study levels of asexual reproduction and genetic variations occurring within and between populations. Stichopus chloronotus is a common holothurian species on Indo-Pacific coral reefs.

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Additional Info

Field Value
Title Population genetics of the fissiparous Holothurian, Stichopus chloronotus, on the Great Barrier Reef
Language eng
Licence Not Specified
Landing Page https://data.gov.au/data/dataset/0e5c70f1-fded-428c-8959-66f8dc7289ff
Contact Point
Australian Ocean Data Network
reception@aims.gov.au
Reference Period 20/11/2017
Geospatial Coverage
Map data © OpenStreetMap contributors
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Data Portal Australian Oceans Data Network

Data Source

This dataset was originally found on Australian Oceans Data Network "Population genetics of the fissiparous Holothurian, Stichopus chloronotus, on the Great Barrier Reef". Please visit the source to access the original metadata of the dataset:
https://catalogue.aodn.org.au/geonetwork/srv/eng/csw/dataset/population-genetics-of-the-fissiparous-holothurian-stichopus-chloronotus-on-the-great-barrier-r1