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Microphytoplankton abundance and composition data collected from the Port Hacking 100m station 1998-2009

Created 23/06/2025

Updated 23/06/2025

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Monthly microphytoplankton (~20 - 200 mm diameter fraction) samples were collected from Port Hacking 100m coastal station by hauling a 20 μm mesh net (245 mm diameter, 1.2 m length) with an attached 120 mL plastic jar (no flow meter) from a depth of 50 m from September 1998 to December 2009. Samples were preserved using 3% glutaraldehyde (final concentration) and stored at 4°C prior to microscopic examination. An Olympus BH2 microscope equipped with Differential Interference Contrast (DIC) and Phase Contrast (≤ 400X magnification) was used to identify and enumerate microphytoplankton taxa. In order to characterise the structure of the microphytoplankton community, species presence/absence and abundance were measured. Species presence/absence was estimated by attempting to identify all microphytoplankton taxa present in each sample to species. For some taxa, species-level identification was not possible using routine light microscopy. In such instances, individuals were identified to genus (e.g. Chaetoceros, Pseudo-nitzschia, and Thalassiosira). The length of time devoted to enumerating taxa in a sample varied from 1 hr to 2 hr, as determined using the timed-interval protocol of the Australian Rivers Assessment System (AUSRIVAS) (http://ausrivas.ewater.com.au). Abundance was estimated by counting to a minimum of 100 cells, or 10 traverses, using the Lund cell method (Hotzel and Croome 1999). Trichodesmium erythraeum filaments were converted to cell counts using a conversion factor (30 cells filament-1) that was obtained by averaging the cell counts of 40 filaments (standard deviation: 6.4 cells filament-1).

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Additional Info

Field Value
Title Microphytoplankton abundance and composition data collected from the Port Hacking 100m station 1998-2009
Language eng
Licence Not Specified
Landing Page https://data.gov.au/data/dataset/8f6ca676-81ad-47f4-85c2-20cd07ccbc00
Contact Point
Australian Ocean Data Network
Penelope.Ajani@uts.edu.au
Reference Period 30/09/1998 - 07/12/2009
Geospatial Coverage
Map data © OpenStreetMap contributors
{
  "coordinates": [
    151.2182,
    -34.1176
  ],
  "type": "Point"
}
Data Portal Australian Oceans Data Network

Data Source

This dataset was originally found on Australian Oceans Data Network "Microphytoplankton abundance and composition data collected from the Port Hacking 100m station 1998-2009". Please visit the source to access the original metadata of the dataset:
https://catalogue.aodn.org.au/geonetwork/srv/eng/csw/dataset/microphytoplankton-abundance-and-composition-data-collected-from-the-port-hacking-100-1998-20091