From Australian Oceans Data Network
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Haemolymph protein in xanthid crabs from the Great Barrier Reef

Created 23/06/2025

Updated 23/06/2025

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Haemolymph was obtained from 26 xanthid crabs collected at low tide from Reef 21-145 and Laver's Cay on the Great Barrier Reef. A combinatin of protease inhibitors (5 mM EDTA, 1 µM pepstatin, 1 µM aprotonin and 100 µM phenylmethylsulfonyl fluoride) was added to the haemolymph. Measurements of equilibrium 3H-STX-binding, time courses of dissociation and association, and the pH-dependence of 3H-STX-binding was conducted on the crabs. Species were: Lophozozymus pictor (2), Liomera tristis (3), Chlorodiella nigra (2), Actaeodes tomentosus (2), Atergatis floridus (15), and Platypodia granulosa (2).Rat-brain synaptosomes were prepared from postmortem rat-brain tissue and binding assays conducted. Tissue extracted from each crab tested for 3H-STX-binding activity was then assayed for paralytic shellfish toxin (PST) activity by rat synaptosome. To report the discovery of a soluble and apparently proteinaceous 3H-STX-binding factor in the haemolymph of several species of xanthid crabs.To describe the attributes of this haemolymph factor in relation to saxiphilin and the sodium channel and to test its ability to bind tetrodotoxin (TTX) and saxitoxin (STX).

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Additional Info

Field Value
Title Haemolymph protein in xanthid crabs from the Great Barrier Reef
Language eng
Licence Not Specified
Landing Page https://data.gov.au/data/dataset/508b3911-2b77-4a58-9d5f-56829883cb14
Contact Point
Australian Ocean Data Network
adc@aims.gov.au
Reference Period 20/11/2017
Geospatial Coverage
Map data © OpenStreetMap contributors
{
  "coordinates": [
    169.26472,
    -19.535
  ],
  "type": "Point"
}
Data Portal Australian Oceans Data Network

Data Source

This dataset was originally found on Australian Oceans Data Network "Haemolymph protein in xanthid crabs from the Great Barrier Reef". Please visit the source to access the original metadata of the dataset:
https://catalogue.aodn.org.au/geonetwork/srv/eng/csw/dataset/haemolymph-protein-in-xanthid-crabs-from-the-great-barrier-reef1