6d272312-1b0d-434b-b18f-3294f29ac964 urn:uuid eng UTF-8 nonGeographicDataset nonGeographicDataset 2025-06-23T15:29:18 creation ISO 19115 2016 Microbial indicators of coral reef ecosystems 6d272312-1b0d-434b-b18f-3294f29ac964 Metagenomic sequencing and 16S rRNA gene sequencing for 381 samples collected over the course of 16 months formed a new comprehensive microbial reference dataset of habitat specific coral reef microbiomes. Originating from samples derived from three locations: Geoffrey Bay, Magnetic Island, QLD (monthly samples) Pioneer Bay, Orpheus Island, QLD (periodic) Channel, Orpheus Island, QLD (periodic) The dataset consists of sequenced bacterial 16S rRNA genes of 381 samples including seawater, sediment, sponge tissue (Coscinoderma matthewsi and Amphimedon queenslandica), coral tissue and mucus (Acropora tenuis and Acropora millepora) and macroalgal surfaces (Sargassum sp.). Water quality parameters and sea surface temperatures were used to compare environmental sensitivity variation in assembly patterns. Results indicated that seawater microbial community data provide diagnostic value to infer shifts in the surrounding reef environment. Sample handling, tissue preparation, DNA extraction procedures and methods of analysis, have been described in associated publications listed below. Accessing the 16S rRNA and metagenome sequences, metadata and protocols are available at the Bioplatforms Australia data portal under the Australian Microbiome project (https://www.bioplatforms.com/australian-microbiome/). Full usage requires free registration. To search for the 16S rRNA sequencing data, navigate to ‘Processed data’, select ‘Amplicon is 27f519r_bacteria’ and ‘Environment is Marine’. Add an additional contextual filter, such as select ‘Sampling Site Location Description’ from the dropdown menu and search for ‘Geoffrey Bay’, 'Pioneer Bay' and 'Channel' or by ‘Sample Type’ i.e. is ‘Seaweed’. {"type": "Point", "coordinates": [146.86466133573853, -19.153205580309955]} 146.86466133573853 146.86466133573853 -19.153205580309955 -19.153205580309955 Glasl, B., Haskell, J. B., Aires, T., Serrão, E. A., Bourne, D. G., Webster, N. S., & Frade, P. R. (2021). Microbial Surface Biofilm Responds to the Growth-Reproduction-Senescence Cycle of the Dominant Coral Reef Macroalgae Sargassum spp. Life, 11(11). https://doi.org/10.3390/life11111199 documentDigital Glasl, B., Haskell, J. B., Aires, T., Serrão, E. A., Bourne, D. G., Webster, N. S., & Frade, P. R. (2021). Microbial Surface Biofilm Responds to the Growth-Reproduction-Senescence Cycle of the Dominant Coral Reef Macroalgae Sargassum spp. Life, 11(11). https://doi.org/10.3390/life11111199 HTML AIMS Water Quality Particulate And Dissolved Nutrient Data documentDigital AIMS Water Quality Particulate And Dissolved Nutrient Data HTML Glasl, B., Robbins, S., Frade, P.R. et al. Comparative genome-centric analysis reveals seasonal variation in the function of coral reef microbiomes. ISME J 14, 1435–1450 (2020). https://doi.org/10.1038/s41396-020-0622-6 documentDigital Glasl, B., Robbins, S., Frade, P.R. et al. Comparative genome-centric analysis reveals seasonal variation in the function of coral reef microbiomes. ISME J 14, 1435–1450 (2020). https://doi.org/10.1038/s41396-020-0622-6 HTML Sea Water Temperature Logger Data At Geoffrey Bay, Great Barrier Reef From 20 Nov 1991 To 10 Jul 2021 documentDigital Sea Water Temperature Logger Data At Geoffrey Bay, Great Barrier Reef From 20 Nov 1991 To 10 Jul 2021 HTML Metagenome assembled genomes (MAGs) are stored at NCBI BioProject PRJNA594068 documentDigital Metagenome assembled genomes (MAGs) are stored at NCBI BioProject PRJNA594068 HTML Glasl, B., Bourne, D.G., Frade, P.R. et al. Microbial indicators of environmental perturbations in coral reef ecosystems. Microbiome 7, 94 (2019). https://doi.org/10.1186/s40168-019-0705-7 documentDigital Glasl, B., Bourne, D.G., Frade, P.R. et al. Microbial indicators of environmental perturbations in coral reef ecosystems. Microbiome 7, 94 (2019). https://doi.org/10.1186/s40168-019-0705-7 HTML Australian Microbiome project -16S rRNA sequencing data - Amplicon 27f519r_bacteria https://www.bioplatforms.com/australian-microbiome/ documentDigital Australian Microbiome project -16S rRNA sequencing data - Amplicon 27f519r_bacteria https://www.bioplatforms.com/australian-microbiome/ HTML AIMS Web Site documentDigital audio/basic AIMS Web Site documentDigital audio/basic AIMS Web Site documentDigital audio/basic AIMS Web Site documentDigital audio/basic Unnamed resource documentDigital License Graphic PNG Unnamed resource documentDigital HTML