d6128949-981f-4bd4-a839-6455d7fb3562 urn:uuid eng UTF-8 nonGeographicDataset nonGeographicDataset 2025-11-17T14:39:01 creation ISO 19115 2016 Cape Darnley Early-Autumn Phytoplankton Bloom, March 2012 d6128949-981f-4bd4-a839-6455d7fb3562 These data relate to a large-scale early-autumn phytoplankton bloom that occurred off Cape Darnley, East Antarctica, in March 2012. The bloom was detected by Dr Jan Lieser (Antarctic Climate and Ecosystems Cooperative Research Centre, ACE-CRC) through MODIS satellite and was opportunistically sampled from RSV Aurora Australis using the uncontaminated seawater line. Samples were analysed for protist species and abundances using light and scanning electron microscopy, and pigment analyses were conducted using high performance liquid chromatography. Additional water samples were taken for dissolved nutrient analyses. Specific details of the files are: Cape Darnley Protist Counts Samples were preserved with 1 % vol:vol Lugols iodine and stored in glass bottles in the dark at 4 degrees C. Protists were identified and counted using phase and Nomarski interference optics using Olympus IX71 and IX81 inverted microscopes at 400X to 640X magnification. Bright field optics were also used to discriminate taxa that contained chloroplasts. Protistan taxa were counted in 20 randomly chosen fields of view, except for highly abundant taxa that were counted in a subset of the field of view defined by an ocular quadrant (Whipple grid). Cell biovolumes and carbon conversion statistics were used to calculate the cell biomass of protistan taxa/groups. Cape Darnley Fluorometer Calibration Fluorometer measurements from the ships underway system were calibrated using chlorophyll a readings determined through high performance liquid chromatography. A linear relationship was established between fluorometer v HPLC chlorophyll a measurements at the same sites. The linear equation was then used to convert all underway fluorometry data from the voyage. Cape Darnley Bloom HPLC Pigments CHEMTAX summary Major phytoplankton groups at each site determined through analysis of pigments using high performance liquid chromatography and CHEMTAX. Methods were according to that of Wright et al. (2010). Cape Darnley Bloom Nutrients Dissolved nutrient concentrations. Samples were analysed by the Department of Primary Industries, Parks, Water and Environment, 18 St. Johns Avenue, Newtown, Tasmania 7008. Cape Darnley Underway Data VOYAGE_04_0_201112 Raw underway data from Aurora Australis in the bloom region Cape Darnley Underway Data Maps Maps of the underway data in the bloom region {"type": "Point", "coordinates": [67.46585, -67.13495]} 67.46585 67.46585 -67.13495 -67.13495 ACE/CRC AMD AMD/AU Bloom CEOS EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > PROTISTS > PLANKTON > PHYTOPLANKTON EARTH SCIENCE > BIOSPHERE > ECOSYSTEMS > AQUATIC ECOSYSTEMS > PLANKTON > PHYTOPLANKTON FLUOROMETERS GEOGRAPHIC REGION > POLAR OCEAN > SOUTHERN OCEAN R/V AA > R/V Aurora Australis SHIPS GET DATA documentDigital Download point for the data HTML VIEW RELATED INFORMATION documentDigital Citation reference for this metadata record and dataset HTML Creative Commons by Attribution logo documentDigital PNG