{"help": "https://data.gov.au/data/en/api/3/action/help_show?name=package_show", "success": true, "result": {"archived": false, "author_email": null, "contact_point": "metadata@aad.gov.au", "creator_user_id": "c2fbbe4a-4ba0-4945-808b-67454605a4cf", "duplicate_score": 1, "geospatial_topic": [], "id": "26b98dca-38e5-4b29-b9a9-a866eef196f1", "isopen": false, "language": "eng", "license_id": "notspecified", "license_title": "notspecified", "maintainer": null, "maintainer_email": null, "metadata_created": "2025-06-23T15:47:10.590047", "metadata_modified": "2025-06-23T15:47:10.590057", "name": "rna-sequence-data-from-krill-co2-exposure-experiment", "notes": "We use RNA sequencing to investigate which genetic/physiological pathways in Antarctic krill are affected by increased CO2 levels. We carried out larval CO2 exposure experiments in March 2012 at the AAD aquarium. Two developmental stages were used (Calyptopis I and Furcilia V) and three CO2 levels (control, 1000 and 2000 ppm).\nThese were short term experiments (2 days) - since initial longer experiments starting with fertilized eggs resulted in differences in developmental stages between treatments and control which could confound the data. RNA was extracted from larvae and high-throughput RNA sequencing (RNA-seq) was carried out on 6 samples (2 stages * 3 treatments). Sequencing was carried out on an Illumina sequencer (Genome Analyzer II). We collected ~ 60 million sequence reads per sample (Data in FASTA format each read gives 100 base pairs of sequence), so a total of ~360 million reads (36 billion bp of data).", "num_resources": 4, "num_tags": 14, "organization": {"id": "0143757a-86ab-43e4-bba6-4a3a2a02b6c4", "name": "australian-ocean-data-network", "title": "Australian Ocean Data Network", "type": "organization", "description": "Harvester for Australian Ocean Data Network", "image_url": "", "created": "2025-06-23T12:29:10.320926", "is_organization": true, "approval_status": "approved", "state": "active"}, "original_harvest_source": {"site_url": "https://catalogue.aodn.org.au", "href": "https://catalogue.aodn.org.au/geonetwork/srv/eng/csw/dataset/rna-sequence-data-from-krill-co2-exposure-experiment", "title": "Australian Oceans Data Network"}, "owner_org": "0143757a-86ab-43e4-bba6-4a3a2a02b6c4", "private": false, "promotion_level": "0", "spatial": "{\"type\": \"Polygon\", \"coordinates\": [[[147.29014, -42.98792], [147.29396, -42.98792], [147.29396, -42.9855], [147.29014, -42.9855], [147.29014, -42.98792]]]}", "spatial_coverage": "{\"type\": \"Polygon\", \"coordinates\": [[[147.29014, -42.98792], [147.29396, -42.98792], [147.29396, -42.9855], [147.29014, -42.9855], [147.29014, -42.98792]]]}", "state": "active", "temporal_coverage_from": "2012-03-01 00:00:00", "temporal_coverage_to": "2012-09-30 00:00:00", "title": "RNA sequence data from krill CO2 exposure experiment", "type": "dataset", "unpublished": false, "url": null, "version": null, "resources": [{"cache_last_updated": null, "cache_url": null, "created": "2025-06-23T15:47:10.593013", "datastore_active": false, "datastore_contains_all_records_of_source_file": false, "description": "Request point for the data", "format": "HTML", "hash": "", "id": "632efe80-b620-4a09-8981-d7a547a831c3", "last_modified": null, "metadata_modified": "2025-06-23T15:47:10.535751", "mimetype": null, "mimetype_inner": null, "name": "GET DATA", "package_id": "26b98dca-38e5-4b29-b9a9-a866eef196f1", "position": 0, "resource_locator_function": "fileAccess", "resource_locator_protocol": "WWW:LINK-1.0-http--link", "resource_type": null, "size": null, "state": "active", "url": "http://data.aad.gov.au/aadc/portal/download_file.cfm?file_id=4618", "url_type": null, "zip_extract": false}, {"cache_last_updated": null, "cache_url": null, "created": "2025-06-23T15:47:10.593020", "datastore_active": false, "datastore_contains_all_records_of_source_file": false, "description": "Public information for AAS project AAS_4015", "format": "HTML", "hash": "", "id": "e8b76720-7374-48f5-b92b-4af33c5e6f39", "last_modified": null, "metadata_modified": "2025-06-23T15:47:10.536030", "mimetype": null, "mimetype_inner": null, "name": "PROJECT HOME PAGE", "package_id": "26b98dca-38e5-4b29-b9a9-a866eef196f1", "position": 1, "resource_locator_function": "fileAccess", "resource_locator_protocol": "WWW:LINK-1.0-http--link", "resource_type": null, "size": null, "state": "active", "url": "https://projects.aad.gov.au/search_projects_results.cfm?project_no=AAS_4015", "url_type": null, "zip_extract": false}, {"cache_last_updated": null, "cache_url": null, "created": "2025-06-23T15:47:10.593022", "datastore_active": false, "datastore_contains_all_records_of_source_file": false, "description": "Citation reference for this metadata record and dataset.", "format": "HTML", "hash": "", "id": "82391709-491d-43e3-8f24-7f7ab9a2f740", "last_modified": null, "metadata_modified": "2025-06-23T15:47:10.536285", "mimetype": null, "mimetype_inner": null, "name": "VIEW RELATED INFORMATION", "package_id": "26b98dca-38e5-4b29-b9a9-a866eef196f1", "position": 2, "resource_locator_function": "fileAccess", "resource_locator_protocol": "WWW:LINK-1.0-http--link", "resource_type": null, "size": null, "state": "active", "url": "https://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_4015_krill_RNA", "url_type": null, "zip_extract": false}, {"cache_last_updated": null, "cache_url": null, "created": "2025-06-23T15:47:10.593025", "datastore_active": false, "datastore_contains_all_records_of_source_file": false, "description": "", "format": "PNG", "hash": "", "id": "109537d1-32b2-4c1d-a2cf-6fc4ede1de5f", "last_modified": null, "metadata_modified": "2025-06-23T15:47:10.536487", "mimetype": null, "mimetype_inner": null, "name": "Creative Commons by Attribution logo", "package_id": "26b98dca-38e5-4b29-b9a9-a866eef196f1", "position": 3, "resource_locator_function": "", "resource_locator_protocol": "", "resource_type": null, "size": null, "state": "active", "url": "https://i.creativecommons.org/l/by/3.0/88x31.png", "url_type": null, "zip_extract": false}], "tags": [{"display_name": "ADS > Automated DNA Sequencer", "id": "10648197-4451-4d77-80e6-ad650a2650f8", "name": "ADS > Automated DNA Sequencer", "state": "active", "vocabulary_id": null}, {"display_name": "AMD", "id": "dba7c99d-b0de-4b01-b166-360aff9b5053", "name": "AMD", "state": "active", "vocabulary_id": null}, {"display_name": "AMD/AU", "id": "3ea7b640-ce0e-4158-9ae0-c1b4c3467b7c", "name": "AMD/AU", "state": "active", "vocabulary_id": null}, {"display_name": "CEOS", "id": "14765c9b-bc4c-4a36-a3a8-5bc635b910a9", "name": "CEOS", "state": "active", "vocabulary_id": null}, {"display_name": "CONTINENT > ANTARCTICA", "id": "3665ce2e-57eb-4d28-9b51-de889028c0c1", "name": "CONTINENT > ANTARCTICA", "state": "active", "vocabulary_id": null}, {"display_name": "CONTINENT > AUSTRALIA/NEW ZEALAND > AUSTRALIA", "id": "b3769ec7-9d5d-463b-ae7e-5819c0b53d8b", "name": "CONTINENT > AUSTRALIA/NEW ZEALAND > AUSTRALIA", "state": "active", "vocabulary_id": null}, {"display_name": "EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > ANIMALS/INVERTEBRATES > ARTHROPODS > CRUSTACEANS > EUPHA", "id": "aa5aad89-b9e6-403e-9d47-1919183b7a4b", "name": "EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > ANIMALS/INVERTEBRATES > ARTHROPODS > CRUSTACEANS > EUPHA", "state": "active", "vocabulary_id": null}, {"display_name": "EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > CARBON DIOXIDE", "id": "755cff79-854f-40a3-8adc-10177a59183c", "name": "EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > CARBON DIOXIDE", "state": "active", "vocabulary_id": null}, {"display_name": "GENETICS", "id": "e96afead-32dc-4c6a-aa40-502048e43000", "name": "GENETICS", "state": "active", "vocabulary_id": null}, {"display_name": "GEOGRAPHIC REGION > POLAR", "id": "3bac0051-c501-49f7-80ab-1484228e2e08", "name": "GEOGRAPHIC REGION > POLAR", "state": "active", "vocabulary_id": null}, {"display_name": "LABORATORY", "id": "5cf666d1-e0bd-4ec8-8ba2-6d59909f474b", "name": "LABORATORY", "state": "active", "vocabulary_id": null}, {"display_name": "OCEAN > SOUTHERN OCEAN", "id": "04d36312-0409-4eb6-b2c9-7fdd86d4df2b", "name": "OCEAN > SOUTHERN OCEAN", "state": "active", "vocabulary_id": null}, {"display_name": "OCEAN ACIDIFICATION", "id": "3ec7b3d3-a5d7-4840-9124-c13d7d8cfe3d", "name": "OCEAN ACIDIFICATION", "state": "active", "vocabulary_id": null}, {"display_name": "RNA", "id": "61c7b0b5-7b2b-4d5f-a695-ee45635d4ab5", "name": "RNA", "state": "active", "vocabulary_id": null}], "groups": [], "relationships_as_subject": [], "relationships_as_object": [], "extras": [{"key": "harvest_object_id", "value": "50521f04-5c0f-49aa-9057-d547fb1aef3d"}, {"key": "harvest_source_id", "value": "a2216e59-91ec-48b1-83cc-d35d9f09fcec"}, {"key": "harvest_source_title", "value": "Australian Oceans Data Network"}]}}