20d577ae-d5bb-4073-b999-dbea160a305d urn:uuid eng UTF-8 nonGeographicDataset nonGeographicDataset 2025-06-23T13:08:48 creation ISO 19115 2016 Spatial metabolomics for symbiotic marine invertebrates 20d577ae-d5bb-4073-b999-dbea160a305d A mass spectrometry imaging workflow was developed for a soft- and hard-bodied cnidarian animal capable of revealing the host and symbiont metabolome in situ, without the need for a priori isotopic labelling or skeleton decalcification. Through the combination of anaesthetizing, embedding, and cryofilm application, the cryosectioning workflow presented produced intact tissue sections for a soft-bodied (sea anemone E. diaphana) and a hard-bodied (coral G. fascicularis) cnidarian in their natural shape for MSI metabolomics or spatial metabolomics. The study used: Soft bodied cnidarians sourced from the GBR - E. diaphana (genotype AIMS4) in symbiosis with the homologous Breviolum minutum (hereafter referred to as B1-anemones, which were inoculated with symbiont culture SCF 127-01) or the heterologous Cladocopium proliferum (C1-anemones) were inoculated with symbiont culture SCF 055-01.10 E. diaphana were sampled 10 mo post-inoculation for MSI and were maintained under ambient temperature of 27°C at 30 μmol m−2 s−1 (12:12 h, light: dark) Hard-bodied cnidarians, the coral G. fascicularis in symbiosis with Cladocopium C40 (ITS2 profile: C40-C3-C115-C40h) was obtained from Palm Islands GBR and kept under ambient temperature of 27°C and at 130–150 μmol m−2 s−1 at full sun (12:12 h light: dark) ITS2 profiles of the symbiont cultures: culture SCF 127-01 - ITS2 profile: B1-B1o-B1p culture SCF 055-01.10 - ITS2 profile: C1-C1b-C1c-C42.2-C1br-C1bh-C1cb-C72k Cladocopium C40: ITS2 profile: C40-C3-C115-C40h {"type": "Point", "coordinates": [147.05520744774122, -19.26720760708421]} 147.05520744774122 147.05520744774122 -19.26720760708421 -19.26720760708421 Wing Yan Chan, David Rudd, & Madeleine JH van Oppen. (2023). Spatial metabolomics for symbiotic marine invertebrates. Life Science Alliance, 6(8), e202301900. https://doi.org/10.26508/lsa.202301900 documentDigital Wing Yan Chan, David Rudd, & Madeleine JH van Oppen. (2023). Spatial metabolomics for symbiotic marine invertebrates. Life Science Alliance, 6(8), e202301900. https://doi.org/10.26508/lsa.202301900 HTML Metabolite annotations on MetaSpace (project name: spatial metabolomics for symbiotic marine invertebrates) https://metaspace2020.eu/ documentDigital Metabolite annotations on MetaSpace (project name: spatial metabolomics for symbiotic marine invertebrates) https://metaspace2020.eu/ HTML AIMS Web Site documentDigital audio/basic AIMS Web Site documentDigital audio/basic AIMS Web Site documentDigital audio/basic AIMS Web Site documentDigital audio/basic Unnamed resource documentDigital License Graphic PNG Unnamed resource documentDigital HTML