731767bf-07cb-438a-9d9e-35410b1c8f26 urn:uuid eng UTF-8 nonGeographicDataset nonGeographicDataset 2026-01-23T06:13:10 creation ISO 19115 2016 Seawater microbiome responses to copper across reef environments 731767bf-07cb-438a-9d9e-35410b1c8f26 This data collection consists of water quality measurements, nutrient data and measured copper concentrations associated with experiments that derived site-specific guidline values (GVs) for tropical seawater microbiomes by integrating 16S rRNA gene amplicon sequencing with quantitative microbiome profiling and concentration-response modelling. Seawater was collected during high tide at three target sites adjacent to the Burdekin region of the Great Barrier Reef, representing varying disturbance histories: (1) Davies Reef, a high ecological value (HEV) coral reef reference site (S 18°49.826’ E 147°38.177’) situated 50 km offshore with low levels of human impacts and part of the Marine Protected Area; (2) Magnetic Island, a slightly to moderately disturbed (SD-MD) inshore coral/algal reef site (S 19°09.279’ E 146°52.108’) with some perturbations from shipping traffic, dredging, industry, and agricultural runoff; (3) Townsville Breakwater in close proximity to the Port of Townsville, a highly disturbed (HD) inshore site (S 19°14.629’ E 146°49.989’) with increased influence from shipping traffic, industry, and urban runoff. Seawater from each site was subsequently used in a 48-h copper exposure experiment. Water quality measurements (dissolved oxygen, pH and salinity) and water samples for nutrients (dissolved organic / inorganic carbon and nitrogen) were taken at 0-h and 48-h, following standard AIMS procedures. Representative water samples from the nominal experimental treatments (0, 0.1, 0.3, 1, 3, 10, 30, 60, 100, 300, 1000, 3000, 8000 μg Cu L-1) were sent to Envirolab for dissolved copper quantification. Data was entered into excel. {"type": "Point", "coordinates": [146.82497978210452, -19.233444421187667]} 146.82497978210452 146.82497978210452 -19.233444421187667 -19.233444421187667 Thomas, M.C., Waugh, G., Vanwonterghem, I., Webster, N.S., Negri, A.P., Luter, H.M. (2025). Defining site-specific water quality guidelines for marine microbiomes under contaminant stress. Environmental Science and Technology. documentDigital Thomas, M.C., Waugh, G., Vanwonterghem, I., Webster, N.S., Negri, A.P., Luter, H.M. (2025). Defining site-specific water quality guidelines for marine microbiomes under contaminant stress. Environmental Science and Technology. HTML Water quality and copper data documentDigital Water quality and copper data XLSX AIMS Web Site documentDigital HTML AIMS Web Site documentDigital HTML AIMS Web Site documentDigital HTML AIMS Web Site documentDigital HTML Unnamed resource documentDigital License Graphic PNG Unnamed resource documentDigital HTML