{"help": "https://data.gov.au/data/api/3/action/help_show?name=package_show", "success": true, "result": {"archived": false, "author_email": null, "contact_point": "metadata@aad.gov.au", "creator_user_id": "c2fbbe4a-4ba0-4945-808b-67454605a4cf", "duplicate_score": 1, "geospatial_topic": [], "id": "cfe0e46a-0f92-495b-8fe3-026d8243766e", "isopen": false, "language": "eng", "license_id": "notspecified", "license_title": "notspecified", "maintainer": null, "maintainer_email": null, "metadata_created": "2025-06-23T09:46:51.612032", "metadata_modified": "2025-06-23T09:46:51.612039", "name": "k-axis-eukaryote-operational-taxonomic-units-otu-table-and-contextual-data", "notes": "Sampling\nSamples were collected on board the RSV Aurora Australis between 22 January and 17 February 2016. The cruise surveyed the region south of the Kerguelen Plateau including the Princess Elizabeth Trough and BANZARE Bank in a series of eight transects covering 8165 km. Plankton communities were collected at 45 conductivity temperature depth (CTD) stations and seven additional underway stations, with biological replicates collected at two stations (52 independent sites). Surface water was sampled from 4 plus or minus 2 m depth using the uncontaminated seawater line. Deep Chlorophyll Maximum (DCM, 10-74 m) water samples were obtained using 10 L Niskin bottles mounted on a Seabird 911+ CTD. Plankton communities were size-fractionated by sequentially filtering 10 L seawater through 25 mm 20 micron (nylon) and 5 micron filters (PVDF), and 0.45 micron Sterivex filters (PVDF). Filters were stored frozen at -80 \u00b0C.\nDNA extraction and high-throughput sequencing\nDNA was extracted from half of each filter using the MoBio PowerSoil DNA Isolation kit at the Australian Genome Research Facility (AGRF, Adelaide, Australia; http://www.agrf.org.au). The V4 region of the 18S rDNA (approximately 380 bp excluding primers) was PCR-amplified using universal eukaryotic primers from all extracts and sequenced on an Illumina MiSeq v2 (2 x 250 bp paired-end) following the Ocean Sampling Day protocol (Piredda et al. 2017). Amplicon library preparation and high-throughput sequencing were carried out at the Ramaciotti Centre for Genomics (Sydney, Australia).\nSequence analysis, OTU picking and assignment followed the Biomes of Australian Soil Environments (BASE) workflow (Bissett et al. 2016). Taxonomy was assigned to OTUs based on the PR2 database using the \u2018classify.seqs\u2019 command in mothur version 1.31.2 with default settings and a bootstrap cut-off of 60%. OTUs representing any terrestrial contaminants (e.g. human) and samples with low sequencing coverage (less than 7000 reads) were removed from the dataset.\nThe date of sea ice melt for each station was estimated from daily SSM/I-derived sea-ice spatial concentration from the National Snow and Ice Data Centre (NSIDC) at 25 x 25 km resolution. Days since melt was considered to be the number of days between the date on which sea ice concentration first fell below 15% and the date of sampling.\nOther environmental variables included are in situ chlorophyll a, as an indicator of biological production, and near-surface salinity (mean over the upper 10 m) as an indicator for recent sea ice melt. Both environmental measurements were taken from the associated CTD seawater samples. The surface chlorophyll a in seawater (1-2 L) collected in Niskin bottles was analysed by high performance liquid chromatography (HPLC, provided by Karen Westwood and Imojen Pearce, Australian Antarctic Division, doi:10.4225/15/5a94c701b98a8).\nSampling times are given in UTC.", "num_resources": 4, "num_tags": 19, "organization": {"id": "0143757a-86ab-43e4-bba6-4a3a2a02b6c4", "name": "australian-ocean-data-network", "title": "Australian Ocean Data Network", "type": "organization", "description": "Harvester for Australian Ocean Data Network", "image_url": "", "created": "2025-06-23T12:29:10.320926", "is_organization": true, "approval_status": "approved", "state": "active"}, "original_harvest_source": {"site_url": "https://catalogue.aodn.org.au", "href": "https://catalogue.aodn.org.au/geonetwork/srv/eng/csw/dataset/k-axis-eukaryote-operational-taxonomic-units-otu-table-and-contextual-data", "title": "Australian Oceans Data Network"}, "owner_org": "0143757a-86ab-43e4-bba6-4a3a2a02b6c4", "private": false, "promotion_level": "0", "spatial": "{\"type\": \"Polygon\", \"coordinates\": [[[68.76833333, -66.6074], [93.57, -66.6074], [93.57, -57.5929], [68.76833333, -57.5929], [68.76833333, -66.6074]]]}", "spatial_coverage": "{\"type\": \"Polygon\", \"coordinates\": [[[68.76833333, -66.6074], [93.57, -66.6074], [93.57, -57.5929], [68.76833333, -57.5929], [68.76833333, -66.6074]]]}", "state": "active", "temporal_coverage_from": "2016-01-22 00:00:00", "temporal_coverage_to": "2016-02-17 00:00:00", "title": "K-Axis eukaryote Operational Taxonomic Units (OTU) table and contextual data", "type": "dataset", "unpublished": false, "url": null, "version": null, "resources": [{"cache_last_updated": null, "cache_url": null, "created": "2025-06-23T09:46:51.616747", "datastore_active": false, "datastore_contains_all_records_of_source_file": false, "description": "Download the dataset", "format": "HTML", "hash": "", "id": "90da2f4a-3c59-445e-97f3-a4e247b18976", "last_modified": null, "metadata_modified": "2025-06-23T09:46:51.578245", "mimetype": null, "mimetype_inner": null, "name": "GET DATA", "package_id": "cfe0e46a-0f92-495b-8fe3-026d8243766e", "position": 0, "resource_locator_function": "fileAccess", "resource_locator_protocol": "WWW:LINK-1.0-http--link", "resource_type": null, "size": null, "state": "active", "url": "https://data.aad.gov.au/eds/4810/download", "url_type": null, "zip_extract": false}, {"cache_last_updated": null, "cache_url": null, "created": "2025-06-23T09:46:51.616752", "datastore_active": false, "datastore_contains_all_records_of_source_file": false, "description": "Public information for AAS project AAS_4313", "format": "HTML", "hash": "", "id": "800a384a-362d-4dc4-98c2-0142a2dad1aa", "last_modified": null, "metadata_modified": "2025-06-23T09:46:51.578413", "mimetype": null, "mimetype_inner": null, "name": "PROJECT HOME PAGE", "package_id": "cfe0e46a-0f92-495b-8fe3-026d8243766e", "position": 1, "resource_locator_function": "fileAccess", "resource_locator_protocol": "WWW:LINK-1.0-http--link", "resource_type": null, "size": null, "state": "active", "url": "https://projects.aad.gov.au/search_projects_results.cfm?project_no=AAS_4313", "url_type": null, "zip_extract": false}, {"cache_last_updated": null, "cache_url": null, "created": "2025-06-23T09:46:51.616753", "datastore_active": false, "datastore_contains_all_records_of_source_file": false, "description": "Citation reference for this metadata record and dataset.", "format": "HTML", "hash": "", "id": "fe6382c7-3001-4102-9f8d-88c1e7966e8e", "last_modified": null, "metadata_modified": "2025-06-23T09:46:51.578524", "mimetype": null, "mimetype_inner": null, "name": "VIEW RELATED INFORMATION", "package_id": "cfe0e46a-0f92-495b-8fe3-026d8243766e", "position": 2, "resource_locator_function": "fileAccess", "resource_locator_protocol": "WWW:LINK-1.0-http--link", "resource_type": null, "size": null, "state": "active", "url": "https://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_4313_KAXIS_EUK_OTUS", "url_type": null, "zip_extract": false}, {"cache_last_updated": null, "cache_url": null, "created": "2025-06-23T09:46:51.616755", "datastore_active": false, "datastore_contains_all_records_of_source_file": false, "description": "", "format": "PNG", "hash": "", "id": "f32a3d5d-f62e-41d8-9587-db3dd1755980", "last_modified": null, "metadata_modified": "2025-06-23T09:46:51.578621", "mimetype": null, "mimetype_inner": null, "name": "Creative Commons by Attribution logo", "package_id": "cfe0e46a-0f92-495b-8fe3-026d8243766e", "position": 3, "resource_locator_function": "", "resource_locator_protocol": "", "resource_type": null, "size": null, "state": "active", "url": "https://i.creativecommons.org/l/by/3.0/88x31.png", "url_type": null, "zip_extract": false}], "tags": [{"display_name": "18S RIBOSOMAL RNA", "id": "21fa274e-5d4a-4db7-bfc6-283dc82af50d", "name": "18S RIBOSOMAL RNA", "state": "active", "vocabulary_id": null}, {"display_name": "ACE/CRC", "id": "0017d8e1-c058-4dd2-bca2-c02e36c1ffed", "name": "ACE/CRC", "state": "active", "vocabulary_id": null}, {"display_name": "ADS > Automated DNA Sequencer", "id": "10648197-4451-4d77-80e6-ad650a2650f8", "name": "ADS > Automated DNA Sequencer", "state": "active", "vocabulary_id": null}, {"display_name": "AMD", "id": "dba7c99d-b0de-4b01-b166-360aff9b5053", "name": "AMD", "state": "active", "vocabulary_id": null}, {"display_name": "AMD/AU", "id": "3ea7b640-ce0e-4158-9ae0-c1b4c3467b7c", "name": "AMD/AU", "state": "active", "vocabulary_id": null}, {"display_name": "CEOS", "id": "14765c9b-bc4c-4a36-a3a8-5bc635b910a9", "name": "CEOS", "state": "active", "vocabulary_id": null}, {"display_name": "CONTINENT > ANTARCTICA", "id": "3665ce2e-57eb-4d28-9b51-de889028c0c1", "name": "CONTINENT > ANTARCTICA", "state": "active", "vocabulary_id": null}, {"display_name": "CTD > Conductivity, Temperature, Depth", "id": "46719d14-fd2f-4dfa-aa7b-7f41ab5cd1c9", "name": "CTD > Conductivity, Temperature, Depth", "state": "active", "vocabulary_id": null}, {"display_name": "EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > PROTISTS > PLANKTON", "id": "1ff87a65-6012-44b7-9233-e9bafe090c32", "name": "EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > PROTISTS > PLANKTON", "state": "active", "vocabulary_id": null}, {"display_name": "EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > CHLOROPHYLL", "id": "d1f851f2-334e-4d05-ab25-d83f1e65ecd6", "name": "EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > CHLOROPHYLL", "state": "active", "vocabulary_id": null}, {"display_name": "EARTH SCIENCE > OCEANS > SALINITY/DENSITY > SALINITY", "id": "6d29b069-f9a6-4a7f-b9ab-1447649c0226", "name": "EARTH SCIENCE > OCEANS > SALINITY/DENSITY > SALINITY", "state": "active", "vocabulary_id": null}, {"display_name": "EUKARYOTE", "id": "42072c20-77b8-4a4e-90cb-96f5d8dafb41", "name": "EUKARYOTE", "state": "active", "vocabulary_id": null}, {"display_name": "GEOGRAPHIC REGION > POLAR", "id": "3bac0051-c501-49f7-80ab-1484228e2e08", "name": "GEOGRAPHIC REGION > POLAR", "state": "active", "vocabulary_id": null}, {"display_name": "ILLUMINA MISEQ", "id": "4fbcfb6f-402e-4508-a5e2-58f528111f6a", "name": "ILLUMINA MISEQ", "state": "active", "vocabulary_id": null}, {"display_name": "LABORATORY", "id": "5cf666d1-e0bd-4ec8-8ba2-6d59909f474b", "name": "LABORATORY", "state": "active", "vocabulary_id": null}, {"display_name": "NISKIN BOTTLES", "id": "4480b231-b278-4747-a217-f9f8dfc835f8", "name": "NISKIN BOTTLES", "state": "active", "vocabulary_id": null}, {"display_name": "OCEAN > SOUTHERN OCEAN > BANZARE BANK", "id": "4a21f350-641b-4556-b57a-b75f62968335", "name": "OCEAN > SOUTHERN OCEAN > BANZARE BANK", "state": "active", "vocabulary_id": null}, {"display_name": "OCEAN > SOUTHERN OCEAN > PRINCESS ELIZABETH TROUGH", "id": "c096a7df-1b7a-44f3-a669-da21ebe95bd9", "name": "OCEAN > SOUTHERN OCEAN > PRINCESS ELIZABETH TROUGH", "state": "active", "vocabulary_id": null}, {"display_name": "R/V AA > R/V Aurora Australis", "id": "de4abe1a-e3d9-441f-9a59-202a7a2ba802", "name": "R/V AA > R/V Aurora Australis", "state": "active", "vocabulary_id": null}], "groups": [], "relationships_as_subject": [], "relationships_as_object": [], "extras": [{"key": "harvest_object_id", "value": "2790ad8f-61b6-4c4c-97ba-5b7499df9e7a"}, {"key": "harvest_source_id", "value": "a2216e59-91ec-48b1-83cc-d35d9f09fcec"}, {"key": "harvest_source_title", "value": "Australian Oceans Data Network"}]}}