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Gene expression in the sponge Rhopaloeides odorabile from the Great Barrier Reef, when thermally stressed

In July 2009, twelve adult Rhopaloeides odorabile were collected at Pelorus Island and cut into a total of 90 clones (approx. 15 cm³) and randomly transferred to plastic oyster racks, which were secured to the reef base with stakes and cable ties. After a 4 month healing period, sponge clones were collected and transported to the indoor aquarium at the Australian Institute of Marine Science (AIMS), where they were acclimated in aquaria at ambient temperature (28°C) for 4 days prior to commencing experiments. Three tanks, each containing 5 clones, were set up for each of three temperature treatments (27°C, 31°C and 32°C, range ± 0.2°C). A single clone was removed from each tank after 0, 1, 3, 7 and 14 days and immediately frozen in liquid nitrogen for molecular analysis.Nine female Rhopaloeides odorabile sponges were collected from Rib Reef in January 2010 and transferred to outdoor aquaria supplied with flow through seawater at AIMS, where they were acclimated for 48 hours. Sponges were then covered with larval collection traps and approximately 4000 larvae were collected and were pooled from all adult individuals. Three temperature controlled tanks were used for each of the 5 temperature treatments (28°C, 30°C, 32°C, 34°C and 36°C, range ± 0.2°C). Five jars, each containing 50 pooled larvae were immersed in each replicate tank. Individual jars were removed after 2, 4, 12, 24 and 48 hours. Time 0 samples of larvae were collected immediately after spawning. Samples were recovered in cryotubes, immediately frozen in liquid nitrogen for preservation of RNA and stored at -80°C for subsequent molecular analysis.A total of 50 mg of adult tissue or 50 individual larvae were used for each mRNA extract. Identical reagents, kits and techniques were used to produce amplicons from both adults and larvae. Protocols for RNA extraction and cDNA preparation were optimized to each sample type.

Data and Resources

Additional Info

Field Value
Title Gene expression in the sponge Rhopaloeides odorabile from the Great Barrier Reef, when thermally stressed
Type Dataset
Language English
Licence Creative Commons Attribution 3.0 Australia
Data Status inactive
Update Frequency notPlanned
Landing Page http://data.gov.au/dataset/bf4a7a4a-f110-44b2-a77d-f272f0931377
Date Published 2017-06-24
Date Updated 2017-10-09
Contact Point
Australian Institute of Marine Science
adc@aims.gov.au
Geospatial Coverage {"type": "Point", "coordinates": [146.487883, -18.545167]}
Jurisdiction Commonwealth of Australia
Data Portal Australian Institute of Marine Science CSW Harvester
Publisher/Agency Australian Institute of Marine Science
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